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KMID : 0894520120160030227
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Development & Reproduction
2012 Volume.16 No. 3 p.227 ~ p.233
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Effect of Reverse Feeding on the Reproductive System in Male Rats
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Jeon Eun-Young
Lee Sung-Ho
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Abstract
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Circadian timing system plays a major role in a wide range of reproductive function. However it is plausible idea that other environmental and/or internal cue might be simultaneously participated in the optimal regulation of reproductive system. In the present study we extended the reverse feeding (RF) time regimen up to 8 weeks, then measured the general and reproductive indices of the animals. The animals of ad libitum feeding group (Control, CON) have free access to food for 4, 6 and 8 weeks, respectively. The day feeding animals (reverse feeding, RF group) have restricted access to food during daytime (09:00-18:00) for 4, 6 and 8 weeks, respectively. When the feeding schedules were over, key indices were measured. After 4 weeks and 8 weeks of feeding, body weights of animals were not significantly different. However, body weights of 6 weeks RF animals were significantly smaller than those of control animals (CON : RF = 333.46±12.71 g : 289.91±8.31 g, p<0.01). The blood glucose levels of 4 weeks RF animals were significantly decreased compared to the levels of control animals (CON : RF = 161.4±2.7 mg/dL : 176.7±5mg/dL, p<0.01) while the levels of 6 weeks RF and 8 weeks RF animals were not different form those of control animals. Reproductive and non-reproductive tissue weights from 6 weeks RF group were significantly lowered than those from CON group (testis, CON : RF = 1.4714±0.0174 g : 1.3724±0.0168 g, p<0.001; epididymis, CON : RF = 0.3574±0.0059 g : 0.3243±0.0068 g, p<0.001; seminal vesicle, CON : RF = 0.1655±0.0068 g : 0.1328±0.0054 g, p<0.001; prostate, CON : RF = 0.3350±0.0231 g : 0.2528±0.0143 g, p<0.01). After 4 weeks and 8 weeks of reverse feeding, sperm counts in RF animals were markedly reduced than those in control animals[CON 4W : RF 4W = 121.17±9.96(x10^6) : 50.86±9(x10^6), p<0.001; CON 8W : RF 8W= 138.69±9.8(x10^6) : 108.94±4.22(x10^6), p<0.001]. Present study indicates that RF may induce an adaptable metabolic stress and cause impairment of androgen-dependent reproductive tissues. On-going longitudinal studies will allow a better understanding of the how does mealtime shift affect the reproductive function and exact nature of adaptation.
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KEYWORD
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Reverse feeding (RF), Male rats, Tissue weight, Blood glucose level, Sperm counts
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